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KMID : 1041220120500020145
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Journal of Sericultural and Entomological Science
2012 Volume.50 No. 2 p.145 ~ p.149
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Isolation and purification of a cecropin-like antimicrobial peptide from the japanese oak silkworm, Antheraea yamamai
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Kim Seong-Ryul
Goo Tae-Won
Choi Kwang-Ho
Park Seung-Won
Kim Sung-Wan
Hwang Jae-Sam
Kang Seok-Woo
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Abstract
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Cecropin is a well-studied antimicrobial peptide that play important role as key factor in insect humoral immunity. In this study, cecropin-like antimicrobial peptide was isolated and purified from the larval haemolymph of immune-challenged japanese oak silkworm, Antheraea yamamai. To isolate antimicrobial peptide, we separated and compared acidic extracted hemolymph protein bends between control and immune-challenged larvae using SDS-PAGE analysis. In the immune hemolymph extract, but not of non-immune hemolymph, we detected differential expressed peptide band with molecular mass 4,223.01 Da. To understand this peptide better, we successfully purified this peptide using cation exchange chromatography and gel permeation chromatography. Its N-terminal amino acid sequence obtained by Edman degradation evidenced a significant degree of identity with other lepidopteran cecropins. The purified A. yamamai cecropin-like peptide showed a broad spectrum of activity against fungi, Gram-negative and Gram-positive bacteria.
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KEYWORD
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Antheraea yamamai, Cecropin, Antimicrobial peptide
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